Hotspot

ycf2-trnL-CAA

IRb · 94655-95356 · 702 bp

Back to Carissa

Pi 0.0057

MarkerSeek score 62.00

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 702
variable_sites: 4
pi: 0.0057
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	811 bp	1.0	78.5
ycf2-trnL-CAA_p2	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	810 bp	1.0	78.5
ycf2-trnL-CAA_p3	ATGGCTTTTCCCGGATGAAA	TGAAATGGTAGACACGCGAG	45.0	50.0	57.8	58.0	813 bp	1.0	78.5
ycf2-trnL-CAA_p4	TGGCTTTTCCCGGATGAAAT	TGAAATGGTAGACACGCGAG	45.0	50.0	57.8	58.0	812 bp	1.0	78.5
ycf2-trnL-CAA_p5	AAGATGGCTTTTCCCGGATG	AAATGGTAGACACGCGAGAC	50.0	50.0	58.2	58.0	814 bp	1.0	78.5