Hotspot

ndhC-trnV-UAC

LSC · 51789-53083 · 1295 bp

Back to Carissa

Pi 0.00154
MarkerSeek score 59.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1295
variable_sites
2
pi
0.00154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1426-1435 bp 1.0 77.4
ndhC-trnV-UAC_p2 CTGGTCCTTTGCTAATCGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.3 57.8 1443-1452 bp 1.0 75.9
ndhC-trnV-UAC_p3 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1438-1447 bp 1.0 75.8
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1425-1434 bp 1.0 74.7
ndhC-trnV-UAC_p5 TCCTTTGCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.5 57.8 1439-1448 bp 1.0 74.6