Hotspot

rpoB-trnC-GCA

LSC · 26969-28232 · 1264 bp

Back to Carissa

Pi 0.00158

MarkerSeek score 59.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1264
variable_sites: 2
pi: 0.00158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGCCAAACCCTGATCAATGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.5	58.1	1422-1437 bp	1.0	75.2
rpoB-trnC-GCA_p2	CGCCAAACCCTGATCAATGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.5	58.4	1404-1419 bp	1.0	73.0
rpoB-trnC-GCA_p3	AAAGTTCTTCCGCCAAACCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.0	58.1	1432-1447 bp	1.0	71.8
rpoB-trnC-GCA_p4	CTCATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	57.9	50.0	58.0	58.1	1347-1362 bp	1.0	71.4
rpoB-trnC-GCA_p5	ATTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1352-1367 bp	1.0	70.8