Hotspot

rps2-rpoC2

LSC · 16398-16611 · 214 bp

Back to Carissa

Pi 0.00467

MarkerSeek score 40.90

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 214
variable_sites: 1
pi: 0.00467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCCTTACGCTTTGCAGAGAT	AGAAGTGCGCTCGATTGATT	50.0	45.0	58.0	58.0	1051-1054 bp	1.0	79.8
rps2-rpoC2_p2	CCCTTACGCTTTGCAGAGAT	CGAGTTTTAGCAAAGGCAGC	50.0	50.0	58.0	58.0	627-630 bp	1.0	79.8
rps2-rpoC2_p3	AACTCCTGCCTCCATCATCT	AGAAGTGCGCTCGATTGATT	50.0	45.0	58.1	58.0	983-986 bp	1.0	79.2
rps2-rpoC2_p4	AACTCCTGCCTCCATCATCT	CGAGTTTTAGCAAAGGCAGC	50.0	50.0	58.1	58.0	559-562 bp	1.0	79.2
rps2-rpoC2_p5	CCCTTACGCTTTGCAGAGAT	TGGGGATTCTTCATTGGTGC	50.0	50.0	58.0	58.1	960-963 bp	1.0	78.7