Hotspot

ccsA

SSC · 112995-113963 · 969 bp

Back to Carduus

Pi 0.00327

MarkerSeek score 52.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 6
pi: 0.00327
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TTCTTAATTAATTTAGGGGATTCC	AGAACCGCGTGAATCACTAC	29.2	50.0	52.5	58.0	1127 bp	1.0	40.4
ccsA_p2	TTTCTTAATTAATTTAGGGGATTCC	AGAACCGCGTGAATCACTAC	28.0	50.0	53.3	58.0	1128 bp	1.0	40.4
ccsA_p3	TTCTTAATTAATTTAGGGGATTCC	TGATTCACATGGTTCTCGCC	29.2	50.0	52.5	58.3	1101 bp	1.0	40.4
ccsA_p4	TTCTTAATTAATTTAGGGGATTCC	GAGAACCGCGTGAATCACTA	29.2	50.0	52.5	57.7	1128 bp	1.0	40.4
ccsA_p5	TTCTTAATTAATTTAGGGGATTCC	GAACCGCGTGAATCACTACA	29.2	50.0	52.5	58.3	1126 bp	1.0	40.4