Hotspot

trnM-CAU-atpE

LSC · 51541-51753 · 213 bp

Back to Carduus

Pi 0.00704

MarkerSeek score 57.40

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 213
variable_sites: 3
pi: 0.00704
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGATGAGTCCCGTTTGGTTT	CAAATCTAGCTCTCCGACGG	50.0	55.0	58.1	57.9	383 bp	1.0	78.9
trnM-CAU-atpE_p2	GGATGAGTCCCGTTTGGTTT	GGCAAATCTAGCTCTCCGAC	50.0	55.0	58.1	58.1	385 bp	1.0	78.7
trnM-CAU-atpE_p3	GGATGAGTCCCGTTTGGTTT	GAGGCAAATCTAGCTCTCCG	50.0	55.0	58.1	57.9	387 bp	1.0	78.7
trnM-CAU-atpE_p4	GGATGAGTCCCGTTTGGTTT	AGGCAAATCTAGCTCTCCGA	50.0	50.0	58.1	58.2	386 bp	1.0	78.1
trnM-CAU-atpE_p5	GGATGAGTCCCGTTTGGTTT	CTAGGACACGAGTAGAGGCT	50.0	55.0	58.1	57.7	362 bp	1.0	77.2