Hotspot

psbK-psbI

LSC · 7778-8181 · 404 bp

Back to Carduus

Pi 0.00248

MarkerSeek score 41.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 404
variable_sites: 2
pi: 0.00248
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	541 bp	1.0	72.4
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	538 bp	1.0	70.4
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	539 bp	1.0	70.4
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	540 bp	1.0	70.4
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGTCCAGGATTACG	50.0	47.6	57.9	57.8	542 bp	1.0	70.0