Hotspot

ndhK

LSC · 47874-48752 · 879 bp

Back to Cardiocrinum

Pi 0.00228

MarkerSeek score 47.00

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 879
variable_sites: 2
pi: 0.00228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	GATTAACCCGCTGTTTTCTGT	TAGAACCCATGGGAGATGCT	42.9	50.0	56.8	57.8	1003 bp	1.0	61.1
ndhK_p2	AACCCGCTGTTTTCTGTTTA	TAGAACCCATGGGAGATGCT	40.0	50.0	55.8	57.8	999 bp	1.0	60.9
ndhK_p3	TAACCCGCTGTTTTCTGTTT	TAGAACCCATGGGAGATGCT	40.0	50.0	55.8	57.8	1000 bp	1.0	60.9
ndhK_p4	TTAACCCGCTGTTTTCTGTT	TAGAACCCATGGGAGATGCT	40.0	50.0	55.8	57.8	1001 bp	1.0	60.9
ndhK_p5	AGATTAACCCGCTGTTTTCTGT	TAGAACCCATGGGAGATGCT	40.9	50.0	58.2	57.8	1004 bp	1.0	60.6