Hotspot

ccsA

SSC · 112427-113392 · 966 bp

Back to Cardiocrinum

Pi 0.00104

MarkerSeek score 47.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGGACACAGCGGATCCTATA	ACATCTGAGATTATGTTCTTATGT	55.0	29.2	58.4	53.6	1076 bp	1.0	40.1
ccsA_p2	TAAAAGGGACACAGCGGATC	ACATCTGAGATTATGTTCTTATGT	50.0	29.2	57.6	53.6	1081 bp	1.0	40.1
ccsA_p3	CTAAAAGGGACACAGCGGAT	ACATCTGAGATTATGTTCTTATGT	50.0	29.2	57.6	53.6	1082 bp	1.0	40.1
ccsA_p4	CTATAATGGAGAACCCCCGC	ACATCTGAGATTATGTTCTTATGT	55.0	29.2	57.5	53.6	1031 bp	1.0	40.1
ccsA_p5	GGGACACAGCGGATCCTATA	ACATCTGAGATTATGTTCTTATGTT	55.0	28.0	58.4	54.3	1076 bp	1.0	40.1