Hotspot

atpF

LSC · 11140-12481 · 1342 bp

Back to Cardiocrinum

Pi 0.00149

MarkerSeek score 59.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1342
variable_sites: 2
pi: 0.00149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CTCGAAGGGTTGCCATTAGT	AAGAAATCGATCAGGGCGAG	50.0	50.0	57.6	57.8	1523-1524 bp	1.0	75.0
atpF_p2	CTCGAAGGGTTGCCATTAGT	GGCGAGTGAGGTGATACAAA	50.0	50.0	57.6	57.6	1504-1505 bp	1.0	73.8
atpF_p3	CTCGAAGGGTTGCCATTAGT	AGGCGAGTGAGGTGATACAA	50.0	50.0	57.6	58.4	1505-1506 bp	1.0	73.3
atpF_p4	ACTAATTTCGTCGGCTCGAA	AAGAAATCGATCAGGGCGAG	45.0	50.0	57.4	57.8	1537-1538 bp	1.0	73.1
atpF_p5	ACTAATTTCGTCGGCTCGAA	GGCGAGTGAGGTGATACAAA	45.0	50.0	57.4	57.6	1518-1519 bp	1.0	71.9