Hotspot

atpA

LSC · 9538-11061 · 1524 bp

Back to Cardiocrinum

Pi 0.00197
MarkerSeek score 59.80
Primer available yes
Label atpA

Feature Metadata

label_name
atpA
feature_type
gene
region
LSC
length_bp
1524
variable_sites
3
pi
0.00197
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA_p1 GGCTATACCAAGAAACCCAT AACGGATTAGCCTTTCGACT 45.0 45.0 54.8 56.9 1695 bp 1.0 45.9
atpA_p2 GGCTATACCAAGAAACCCAT ACGGATTAGCCTTTCGACTT 45.0 45.0 54.8 56.9 1694 bp 1.0 45.9
atpA_p3 GGCTATACCAAGAAACCCATT AACGGATTAGCCTTTCGACT 42.9 45.0 55.6 56.9 1695 bp 1.0 44.6
atpA_p4 GGCTATACCAAGAAACCCATT ACGGATTAGCCTTTCGACTT 42.9 45.0 55.6 56.9 1694 bp 1.0 44.6
atpA_p5 GGCTATACCAAGAAACCCAT ACGGATTAGCCTTTCGACTTT 45.0 42.9 54.8 57.7 1694 bp 1.0 43.7