Hotspot

rps15-ycf1

SSC · 121314-121712 · 399 bp

Back to Cardiocrinum

Pi 0.0201

MarkerSeek score 66.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 399
variable_sites: 8
pi: 0.0201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAAATGCGAGGTGAGTCTCC	ACCCATAACAGCAGTCGTTT	55.0	45.0	58.6	57.4	584-585 bp	1.0	71.6
rps15-ycf1_p2	TCGTCTTGCGGAATAACTGA	ACCCATAACAGCAGTCGTTT	45.0	45.0	57.0	57.4	505-506 bp	1.0	68.8
rps15-ycf1_p3	TCTTCTTTTTCGTCTTGCGGA	ACCCATAACAGCAGTCGTTT	42.9	45.0	58.2	57.4	514-515 bp	1.0	67.5
rps15-ycf1_p4	CAAATGCGAGGTGAGTCTCC	ACCCATAACAGCAGTCGTTTT	55.0	42.9	58.6	58.1	584-585 bp	1.0	67.0
rps15-ycf1_p5	CGAGGTGAGTCTCCGTATTT	ACCCATAACAGCAGTCGTTT	50.0	45.0	56.8	57.4	578-579 bp	1.0	66.9