Hotspot

petA-psaJ

LSC · 61173-61973 · 801 bp

Back to Cardiocrinum

Pi 0.00125

MarkerSeek score 59.60

Primer available yes

Label petA-psaJ

Feature Metadata

label_name: petA-psaJ
feature_type: igs
region: LSC
length_bp: 801
variable_sites: 1
pi: 0.00125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psaJ_p1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.0	58.3	952-953 bp	1.0	77.4
petA-psaJ_p2	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.0	57.6	948-949 bp	1.0	76.4
petA-psaJ_p3	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	50.0	50.0	58.0	57.6	951-952 bp	1.0	76.4
petA-psaJ_p4	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	949-950 bp	1.0	76.2
petA-psaJ_p5	CATTACGTGTCCAAGGCCTT	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.2	57.6	945-946 bp	1.0	75.3