Hotspot

ycf4-cemA

LSC · 58541-59289 · 749 bp

Back to Cardiocrinum

Pi 0.00401

MarkerSeek score 61.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 3
pi: 0.00401
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGCGTGTACCAATCGAAGT	TGAAAGAGAGACCCACCAGG	45.0	55.0	57.8	58.6	847-848 bp	1.0	73.9
ycf4-cemA_p2	AAAAGCTGCCGAATTAGCCT	TGAAAGAGAGACCCACCAGG	45.0	55.0	58.2	58.6	872-873 bp	1.0	73.9
ycf4-cemA_p3	CCTTGACTGGCACTGATGAG	TGAAAGAGAGACCCACCAGG	55.0	55.0	58.3	58.6	918-919 bp	1.0	73.0
ycf4-cemA_p4	TCTTGCGTGTACCAATCGAA	TGAAAGAGAGACCCACCAGG	45.0	55.0	57.6	58.6	849-850 bp	1.0	71.7
ycf4-cemA_p5	TTCTTGCGTGTACCAATCGA	TGAAAGAGAGACCCACCAGG	45.0	55.0	57.6	58.6	850-851 bp	1.0	71.7