Hotspot

trnD-GUC-trnY-GUA

LSC · 29328-29649 · 322 bp

Back to Cardiocrinum

Pi 0.00314

MarkerSeek score 49.60

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 322
variable_sites: 1
pi: 0.00314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GGATCCTAGTTCGGGACTGA	AATTCTTCCTGGGTCGATGC	55.0	50.0	57.9	58.0	498-501 bp	1.0	79.9
trnD-GUC-trnY-GUA_p2	GTGCTCTGACCGATTGAACT	AATTCTTCCTGGGTCGATGC	50.0	50.0	57.9	58.0	441-444 bp	1.0	79.9
trnD-GUC-trnY-GUA_p3	GGATCCTAGTTCGGGACTGA	TGGCAATATGTCTACGCTGG	55.0	50.0	57.9	57.8	444-447 bp	1.0	78.4
trnD-GUC-trnY-GUA_p4	GGATCCTAGTTCGGGACTGA	GGCAATATGTCTACGCTGGT	55.0	50.0	57.9	57.8	443-446 bp	1.0	78.4
trnD-GUC-trnY-GUA_p5	GTGCTCTGACCGATTGAACT	TGGCAATATGTCTACGCTGG	50.0	50.0	57.9	57.8	387-390 bp	1.0	78.4