Hotspot

psbM-trnD-GUC

LSC · 28078-29253 · 1176 bp

Back to Cardiocrinum

Pi 0.000855

MarkerSeek score 46.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1176
variable_sites: 1
pi: 0.000855
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GCGAGAATATTGACTTCCAT	CCTCGCTGGGATTGTAGTTC	40.0	55.0	53.2	58.1	1270-1277 bp	1.0	41.5
psbM-trnD-GUC_p2	GCGAGAATATTGACTTCCAT	AGTTCAATCGGTCAGAGCAC	40.0	50.0	53.2	57.9	1255-1262 bp	1.0	41.3
psbM-trnD-GUC_p3	GCGAGAATATTGACTTCCAT	TACACCTCGCTGGGATTGTA	40.0	50.0	53.2	58.1	1274-1281 bp	1.0	40.8
psbM-trnD-GUC_p4	GCGAGAATATTGACTTCCAT	CATACACCTCGCTGGGATTG	40.0	55.0	53.2	58.4	1276-1283 bp	1.0	40.5
psbM-trnD-GUC_p5	GCGAGAATATTGACTTCCAT	GTATGCCATACACCTCGCTG	40.0	55.0	53.2	58.5	1282-1289 bp	1.0	40.5