Hotspot

atpI-rps2

LSC · 14849-15114 · 266 bp

Back to Cardiocrinum

Pi 0.00752

MarkerSeek score 56.80

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 266
variable_sites: 2
pi: 0.00752
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	ATGTTGGCCCACTTCTACAC	TGCGAGGGTCGTTATAGCTA	50.0	50.0	57.8	58.0	377 bp	1.0	78.6
atpI-rps2_p2	ATGTTGGCCCACTTCTACAC	TTTGCGAGGGTCGTTATAGC	50.0	50.0	57.8	58.1	379 bp	1.0	78.3
atpI-rps2_p3	GCCCACTTCTACACCCGATA	TGCGAGGGTCGTTATAGCTA	55.0	50.0	58.9	58.0	371 bp	1.0	73.1
atpI-rps2_p4	GCCCACTTCTACACCCGATA	TTTGCGAGGGTCGTTATAGC	55.0	50.0	58.9	58.1	373 bp	1.0	72.8
atpI-rps2_p5	AATGTTGGCCCACTTCTACA	TGCGAGGGTCGTTATAGCTA	45.0	50.0	57.0	58.0	378 bp	1.0	72.4