Hotspot

trnS-GCU-trnG-GCC

LSC · 7748-8443 · 696 bp

Back to Cardiocrinum

Pi 0.00144

MarkerSeek score 59.60

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 1
pi: 0.00144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	TGTCGGAAACTGAAAGACCC	45.0	50.0	58.0	57.8	815 bp	1.0	77.8
trnS-GCU-trnG-GCC_p2	ATTAGCAATCCGCCGCTTTA	CGGAAACTGAAAGACCCCTT	45.0	50.0	58.0	57.7	812 bp	1.0	77.6
trnS-GCU-trnG-GCC_p3	TGATGAGGGATAGCGGAGAG	TGTCGGAAACTGAAAGACCC	55.0	50.0	58.1	57.8	878 bp	1.0	77.4
trnS-GCU-trnG-GCC_p4	TGATGAGGGATAGCGGAGAG	CGGAAACTGAAAGACCCCTT	55.0	50.0	58.1	57.7	875 bp	1.0	77.2
trnS-GCU-trnG-GCC_p5	GAGGGATAGCGGAGAGAGAG	TGTCGGAAACTGAAAGACCC	60.0	50.0	58.2	57.8	874 bp	1.0	76.7