Hotspot

rps16-trnQ-UUG

LSC · 5938-6724 · 787 bp

Back to Cardiocrinum

Pi 0.00254

MarkerSeek score 56.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 2
pi: 0.00254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	860-866 bp	1.0	78.5
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	871-877 bp	1.0	77.9
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	864-870 bp	1.0	77.4
rps16-trnQ-UUG_p4	GGCGGATGTAAGAATCCACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	896-902 bp	1.0	77.0
rps16-trnQ-UUG_p5	TGGCGGATGTAAGAATCCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	897-903 bp	1.0	77.0