Hotspot

ycf1

SSC · 124545-129938 · 5394 bp

Back to Cardamine

Pi 0.0225

MarkerSeek score 68.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5394
variable_sites: 650
pi: 0.0225
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAGACAAACGGACAGGTTC	GCGTGGGCTAGATTAGGAAC	50.0	55.0	58.1	58.1	1154-1169 bp	0.84	80.5
ycf1_p2	CAGACAAACGGACAGGTTCA	GCGTGGGCTAGATTAGGAAC	50.0	55.0	58.1	58.1	1153-1168 bp	0.84	80.5
ycf1_p3	ACCGGCCGATTCTTTTTCTT	GCGTGGGCTAGATTAGGAAC	45.0	55.0	58.1	58.1	756-771 bp	0.84	80.4
ycf1_p4	GTATGACCCACGGCTTCATT	GCGTGGGCTAGATTAGGAAC	50.0	55.0	58.0	58.1	616-625 bp	0.84	80.2
ycf1_p5	ATGACCCACGGCTTCATTTT	GCGTGGGCTAGATTAGGAAC	45.0	55.0	58.1	58.1	614-623 bp	0.84	79.9