Hotspot

rbcL-accD

LSC · 56582-57262 · 681 bp

Back to Cardamine

Pi 0.021

MarkerSeek score 81.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 681
variable_sites: 73
pi: 0.021
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	TAGCTCGCCTCTGTATTCCA	55.0	50.0	57.7	57.9	821-853 bp	0.96	86.1
rbcL-accD_p2	AACTTCCCAACCGTCGATAC	TAGCTCGCCTCTGTATTCCA	50.0	50.0	57.6	57.9	772-804 bp	0.96	86.0
rbcL-accD_p3	TGAGGCTTGCAAATGGAGTC	TAGCTCGCCTCTGTATTCCA	50.0	50.0	58.5	57.9	836-868 bp	0.96	84.6
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	GCTCGCCTCTGTATTCCAAT	55.0	50.0	57.7	57.8	819-851 bp	0.96	84.6
rbcL-accD_p5	GCTTGCAAATGGAGTCCTGA	TAGCTCGCCTCTGTATTCCA	50.0	50.0	58.5	57.9	832-864 bp	0.92	82.8