Hotspot

ndhC-trnV-UAC

LSC · 50470-51386 · 917 bp

Back to Cardamine

Pi 0.0337
MarkerSeek score 79.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
917
variable_sites
155
pi
0.0337
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.95
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAACAGGAATAGCACT GTTCGAGTCCGTATAGCCCT 42.9 55.0 58.1 58.7 903-1012 bp 1.0 77.5
ndhC-trnV-UAC_p2 TGCCAAAACAGGAATAGCACT TTCGAGTCCGTATAGCCCTA 42.9 50.0 58.1 57.1 902-1011 bp 1.0 75.5
ndhC-trnV-UAC_p3 TGCCAAAACAGGAATAGCAC GTTCGAGTCCGTATAGCCCT 45.0 55.0 56.6 58.7 903-1012 bp 1.0 75.4
ndhC-trnV-UAC_p4 TGCCAAAACAGGAATAGCACT TCGAACTCTTTTACCGAGCAG 42.9 47.6 58.1 58.1 931-1040 bp 1.0 74.5
ndhC-trnV-UAC_p5 TGCCAAAACAGGAATAGCACT TCGAACTCTTTTACCGAGCA 42.9 45.0 58.1 56.9 931-1040 bp 1.0 74.3