Hotspot

trnF-GAA-ndhJ

LSC · 48179-48780 · 602 bp

Back to Cardamine

Pi 0.0245

MarkerSeek score 78.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 602
variable_sites: 84
pi: 0.0245
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAACGGAT	50.0	50.0	58.1	58.0	526-758 bp	1.0	91.5
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	499-731 bp	1.0	91.3
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	498-730 bp	1.0	91.3
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCTAATGCCCGAAAGTTGGA	50.0	50.0	58.1	57.9	506-738 bp	1.0	90.6
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TCCTAATGCCCGAAAGTTGG	50.0	50.0	58.1	57.9	507-739 bp	1.0	90.6