Hotspot

trnL-UAA-trnF-GAA

LSC · 47574-48105 · 532 bp

Back to Cardamine

Pi 0.042

MarkerSeek score 74.20

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 532
variable_sites: 92
pi: 0.042
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	435-836 bp	1.0	87.8
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	432-833 bp	1.0	84.5
trnL-UAA-trnF-GAA_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	424-825 bp	1.0	83.9
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	279-708 bp	1.0	83.4
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	50.0	55.0	57.5	58.6	440-798 bp	0.96	81.9