Hotspot

trnT-UGU-trnL-UAA

LSC · 46292-46978 · 687 bp

Back to Cardamine

Pi 0.0264

MarkerSeek score 79.00

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 105
pi: 0.0264
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	740-791 bp	1.0	88.9
trnT-UGU-trnL-UAA_p2	TATGGAAAAGCCGGCTATCG	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	760-810 bp	0.96	87.9
trnT-UGU-trnL-UAA_p3	TATGGAAAAGCCGGCTATCG	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	763-813 bp	0.96	87.2
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	55.0	55.0	57.9	58.2	708-754 bp	0.92	86.5
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	711-757 bp	0.92	85.8