Hotspot

rps4-trnT-UGU

LSC · 45833-46218 · 386 bp

Back to Cardamine

Pi 0.0194

MarkerSeek score 76.80

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 49
pi: 0.0194
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CGGAGCGTGATTGGTTTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	522-540 bp	1.0	89.5
rps4-trnT-UGU_p2	TCTAAGATCGCTTCCTGCCT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.2	57.8	506-524 bp	1.0	87.9
rps4-trnT-UGU_p3	CGGAGCGTGATTGGTTTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	554-572 bp	0.92	84.8
rps4-trnT-UGU_p4	TCTAAGATCGCTTCCTGCCT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.2	57.9	538-556 bp	0.92	83.2
rps4-trnT-UGU_p5	CGGAGCGTGATTGGTTTCTA	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.0	57.5	562-580 bp	0.92	81.7