Hotspot

trnE-UUC-trnT-GGU

LSC · 30612-31429 · 818 bp

Back to Cardamine

Pi 0.0293

MarkerSeek score 81.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 135
pi: 0.0293
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	586-980 bp	1.0	90.7
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	565-959 bp	1.0	90.2
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	562-956 bp	1.0	90.0
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	548-942 bp	1.0	89.8
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	527-921 bp	1.0	89.7