Hotspot

trnG-UCC-trnR-UCU

LSC · 9375-9533 · 159 bp

Back to Cardamine

Pi 0.0417

MarkerSeek score 76.30

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 159
variable_sites: 36
pi: 0.0417
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	277-289 bp	1.0	58.8
trnG-UCC-trnR-UCU_p2	CGACGTCGACTAAAACCCTT	TGATATTGCGTCCAATAGGA	50.0	40.0	57.9	53.9	291-303 bp	1.0	58.4
trnG-UCC-trnR-UCU_p3	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	50.0	40.0	58.1	53.9	279-291 bp	1.0	58.4
trnG-UCC-trnR-UCU_p4	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	271-283 bp	0.96	57.3
trnG-UCC-trnR-UCU_p5	AGCCTTCCAAGCTAACGATG	TTGATATTGCGTCCAATAGGA	50.0	38.1	58.0	54.8	272-284 bp	0.96	56.3