Hotspot

psbK-psbI

LSC · 7202-7619 · 418 bp

Back to Cardamine

Pi 0.0352

MarkerSeek score 82.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 418
variable_sites: 65
pi: 0.0352
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCTGTAAGTTTTCG	TTCTTCACGTCCCGGATTAC	50.0	50.0	58.0	57.3	417-561 bp	1.0	86.3
psbK-psbI_p2	AGCCGCTGTAAGTTTTCGAT	TTCTTCACGTCCCGGATTAC	45.0	50.0	57.9	57.3	415-559 bp	1.0	86.0
psbK-psbI_p3	AAGCCGCTGTAAGTTTTCGA	TTCTTCACGTCCCGGATTAC	45.0	50.0	58.1	57.3	416-560 bp	1.0	85.7
psbK-psbI_p4	GCCGCTGTAAGTTTTCGATG	TTCTTCACGTCCCGGATTAC	50.0	50.0	57.8	57.3	414-558 bp	1.0	85.4
psbK-psbI_p5	GCAAGCCGCTGTAAGTTTTC	TTCTTCACGTCCCGGATTAC	50.0	50.0	58.3	57.3	418-562 bp	1.0	84.2