Hotspot

ndhF

SSC · 116692-118956 · 2265 bp

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Pi 0.437

MarkerSeek score 78.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2265
variable_sites: 827
pi: 0.437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	AACCACTTCCGACTTCTTGA	50.0	45.0	58.0	56.7	2885 bp	0.5	49.5
ndhF_p2	ACGGAAGAAAGGGAGGAAGA	AACCACTTCCGACTTCTTGA	50.0	45.0	58.0	56.7	2936 bp	0.5	49.4
ndhF_p3	GCAGAATACCGTCACCCATT	AACCACTTCCGACTTCTTGA	50.0	45.0	58.0	56.7	2986 bp	0.5	49.3
ndhF_p4	GATCCGGACAAAATCGACGA	AACCACTTCCGACTTCTTGA	50.0	45.0	58.1	56.7	2807 bp	0.5	49.0
ndhF_p5	AGGGGACTAAACAGGAACCA	AACCACTTCCGACTTCTTGA	50.0	45.0	57.9	56.7	2881 bp	0.5	48.6