Hotspot

ndhA

SSC · 127002-129150 · 2149 bp

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Pi 0.485

MarkerSeek score 78.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2149
variable_sites: 858
pi: 0.485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCAATGTACCTTGCAGCTC	ACCTCATGGCTGGATCGATA	50.0	50.0	58.2	58.0	2989 bp	0.5	58.5
ndhA_p2	ACCAATGTACCTTGCAGCTC	TACCTCATGGCTGGATCGAT	50.0	50.0	58.2	58.0	2990 bp	0.5	58.5
ndhA_p3	GACCAATGTACCTTGCAGCT	ACCTCATGGCTGGATCGATA	50.0	50.0	58.2	58.0	2990 bp	0.5	58.5
ndhA_p4	GACCAATGTACCTTGCAGCT	TACCTCATGGCTGGATCGAT	50.0	50.0	58.2	58.0	2991 bp	0.5	58.5
ndhA_p5	ACCAGTTACCATAGGGAGCA	ACCTCATGGCTGGATCGATA	50.0	50.0	57.7	58.0	2941 bp	0.5	57.8