Hotspot

rps15-ycf1

SSC · 130709-131158 · 450 bp

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Pi 0.477

MarkerSeek score 80.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 450
variable_sites: 205
pi: 0.477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TTGAAATTCGACGGATCCCC	GCGAGTCGGTTCAGTATGTT	50.0	50.0	58.0	58.0	573 bp	0.5	59.7
rps15-ycf1_p2	GATGAGTCGAAGAACGCCAT	GCGAGTCGGTTCAGTATGTT	50.0	50.0	58.1	58.0	969 bp	0.5	59.4
rps15-ycf1_p3	CCGTTGGCGTTTTCCTAGTA	GCGAGTCGGTTCAGTATGTT	50.0	50.0	57.9	58.0	684 bp	0.5	59.3
rps15-ycf1_p4	TGACTCCCGAATATCCAGCT	GCGAGTCGGTTCAGTATGTT	50.0	50.0	57.9	58.0	759 bp	0.5	59.2
rps15-ycf1_p5	TCCGCCATAAAAGGACCAAG	GCGAGTCGGTTCAGTATGTT	50.0	50.0	57.9	58.0	1253 bp	0.5	59.0