Hotspot

ndhH-rps15

SSC · 130334-130444 · 111 bp

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Pi 0.412

MarkerSeek score 76.50

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 111
variable_sites: 42
pi: 0.412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	ATCGATCCAGCCATGAGGTA	GGGGATCCGTCGAATTTCAA	50.0	50.0	58.0	58.0	848 bp	0.5	59.6
ndhH-rps15_p2	TATCGATCCAGCCATGAGGT	GGGGATCCGTCGAATTTCAA	50.0	50.0	58.0	58.0	849 bp	0.5	59.6
ndhH-rps15_p3	ATCGATCCAGCCATGAGGTA	TACTAGGAAAACGCCAACGG	50.0	50.0	58.0	57.9	737 bp	0.5	59.2
ndhH-rps15_p4	TATCGATCCAGCCATGAGGT	TACTAGGAAAACGCCAACGG	50.0	50.0	58.0	57.9	738 bp	0.5	59.2
ndhH-rps15_p5	ATCGATCCAGCCATGAGGTA	AGCTGGATATTCGGGAGTCA	50.0	50.0	58.0	57.9	662 bp	0.5	59.2