Hotspot

ndhG-ndhI

SSC · 126014-126415 · 402 bp

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Pi 0.433

MarkerSeek score 78.60

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 402
variable_sites: 167
pi: 0.433
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CACCCGGTTGGATTGACTAG	TGCTCCCTATGGTAACTGGT	55.0	50.0	58.0	57.7	1621 bp	0.5	57.7
ndhG-ndhI_p2	ACCCGGTTGGATTGACTAGA	TGCTCCCTATGGTAACTGGT	50.0	50.0	58.1	57.7	1620 bp	0.5	57.5
ndhG-ndhI_p3	CACCCGGTTGGATTGACTAG	GATCACCTTATCCCACGCAA	55.0	50.0	58.0	57.7	1542 bp	0.5	57.2
ndhG-ndhI_p4	CACCCGGTTGGATTGACTAG	ACCTTATCCCACGCAAATCG	55.0	50.0	58.0	58.3	1538 bp	0.5	57.1
ndhG-ndhI_p5	GCAATCGCACCTATCAAAGC	TGCTCCCTATGGTAACTGGT	50.0	50.0	57.9	57.7	1462 bp	0.5	57.0