Hotspot

ndhE-ndhG

SSC · 125235-125434 · 200 bp

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Pi 0.445

MarkerSeek score 76.20

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 200
variable_sites: 81
pi: 0.445
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	ATAGCCAACCCAATAGCTGC	GCTTTGATAGGTGCGATTGC	50.0	50.0	58.0	57.9	472 bp	0.5	59.0
ndhE-ndhG_p2	ATAGCCAACCCAATAGCTGC	GTTGGGGATGGGGTTACTTC	50.0	55.0	58.0	57.9	691 bp	0.5	58.8
ndhE-ndhG_p3	ATAGCCAACCCAATAGCTGC	TAAGTTTTTCCCGGTCGGAC	50.0	50.0	58.0	57.8	1045 bp	0.5	58.5
ndhE-ndhG_p4	ATAGCCAACCCAATAGCTGC	TTGCTGTGGCTCGTCAATAA	50.0	45.0	58.0	57.8	456 bp	0.5	58.5
ndhE-ndhG_p5	GTTGGACAGGCAGATTCACA	GCTTTGATAGGTGCGATTGC	50.0	50.0	58.1	57.9	966 bp	0.5	58.3