Hotspot

ndhD-psaC

SSC · 124293-124437 · 145 bp

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Pi 0.533

MarkerSeek score 76.90

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 145
variable_sites: 72
pi: 0.533
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGAACCTGCGGATATCGGTA	GCAGCTATTGGGTTGGCTAT	50.0	50.0	58.0	58.0	759 bp	0.5	59.8
ndhD-psaC_p2	AGAACCTGCGGATATCGGTA	GGCATGAAACAACTCGAAGC	50.0	50.0	58.0	58.0	216 bp	0.5	59.7
ndhD-psaC_p3	GCGTATCTGGCAACCATGTA	GCAGCTATTGGGTTGGCTAT	50.0	50.0	58.1	58.0	1414 bp	0.5	59.5
ndhD-psaC_p4	GCGTATCTGGCAACCATGTA	GGCATGAAACAACTCGAAGC	50.0	50.0	58.1	58.0	871 bp	0.5	59.3
ndhD-psaC_p5	TTACGAGTAACTGGCCAAGC	GCAGCTATTGGGTTGGCTAT	50.0	50.0	57.9	58.0	1031 bp	0.5	59.2