Hotspot

ccsA-ndhD

SSC · 122539-122807 · 269 bp

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Pi 0.494

MarkerSeek score 76.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 269
variable_sites: 123
pi: 0.494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTTGCTACTTCGGGGCTTTT	TTACGCCAGATGTTCCATGG	45.0	50.0	58.0	58.0	1103 bp	0.5	59.6
ccsA-ndhD_p2	TTTTGCTACTTCGGGGCTTT	TTACGCCAGATGTTCCATGG	45.0	50.0	58.0	58.0	1104 bp	0.5	59.6
ccsA-ndhD_p3	TTTGCTACTTCGGGGCTTTT	TTGGCAGGAACGAGTTTTGA	45.0	45.0	58.0	58.0	1370 bp	0.5	59.6
ccsA-ndhD_p4	TTTTGCTACTTCGGGGCTTT	TTGGCAGGAACGAGTTTTGA	45.0	45.0	58.0	58.0	1371 bp	0.5	59.6
ccsA-ndhD_p5	TTTGCTACTTCGGGGCTTTT	TATCTTGACGAAATGGGCGG	45.0	50.0	58.0	58.1	1334 bp	0.5	59.5