Hotspot

rpl32-trnL-UAG

SSC · 119994-121379 · 1386 bp

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Pi 0.449

MarkerSeek score 80.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1386
variable_sites: 520
pi: 0.449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CAGCCTTGAAAGCTTTGTCG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1510 bp	0.5	59.6
rpl32-trnL-UAG_p2	CAGCCTTGAAAGCTTTGTCG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1511 bp	0.5	59.6
rpl32-trnL-UAG_p3	CAGCCTTGAAAGCTTTGTCG	AAAAGCCCCGAAGTAGCAAA	50.0	45.0	58.0	58.0	2027 bp	0.5	59.6
rpl32-trnL-UAG_p4	CAGCCTTGAAAGCTTTGTCG	AAAGCCCCGAAGTAGCAAAA	50.0	45.0	58.0	58.0	2026 bp	0.5	59.6
rpl32-trnL-UAG_p5	GGATATTGGGCAGCCTTGAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	1520 bp	0.5	59.4