Hotspot

clpP

Genome · 71173-72505 · 1333 bp

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Pi 0.0641

MarkerSeek score 77.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: Genome
length_bp: 1333
variable_sites: 313
pi: 0.0641
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCCAATCTAGCTGGGTCCAA	AACAGGGTCAGAGGGATCAA	50.0	50.0	58.0	58.0	2172 bp	0.043478	41.1
clpP_p2	TCCAATCTAGCTGGGTCCAA	ACAGGGTCAGAGGGATCAAA	50.0	50.0	58.0	58.0	2171 bp	0.043478	41.1
clpP_p3	TTCCAATCTAGCTGGGTCCA	AACAGGGTCAGAGGGATCAA	50.0	50.0	58.0	58.0	2173 bp	0.043478	41.1
clpP_p4	TTCCAATCTAGCTGGGTCCA	ACAGGGTCAGAGGGATCAAA	50.0	50.0	58.0	58.0	2172 bp	0.043478	41.1
clpP_p5	TCCAATCTAGCTGGGTCCAA	ACGAACATACCCTGTCTCCA	50.0	50.0	58.0	58.1	2204 bp	0.043478	40.9