Hotspot

trnN-GUU-ycf1

Genome · 111489-112248 · 760 bp

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Pi 0.173

MarkerSeek score 74.20

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: Genome
length_bp: 760
variable_sites: 328
pi: 0.173
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	ACCGAATAGCAGAGCAAGTG	CCTTTCTTCCATAACCCGGG	50.0	55.0	58.0	57.9	913-1695 bp	0.956522	89.7
trnN-GUU-ycf1_p2	ACCGAATAGCAGAGCAAGTG	ACGGTAGAGTTAGGGCAGTT	50.0	50.0	58.0	58.1	1043-1825 bp	0.956522	89.6
trnN-GUU-ycf1_p3	CGGGAGAATTGATGACTGCA	CCTTTCTTCCATAACCCGGG	50.0	55.0	58.0	57.9	961-1605 bp	0.913043	87.9
trnN-GUU-ycf1_p4	CGGGAGAATTGATGACTGCA	ACGGTAGAGTTAGGGCAGTT	50.0	50.0	58.0	58.1	1091-1735 bp	0.913043	87.8
trnN-GUU-ycf1_p5	ACCGACTAAAAGGGGAAGGA	CCTTTCTTCCATAACCCGGG	50.0	55.0	58.0	57.9	1289-1950 bp	0.73913	80.9