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rps19-rpl2

Genome · 85370-87435 · 2066 bp

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Pi 0.346

MarkerSeek score 77.00

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: Genome
length_bp: 2066
variable_sites: 1388
pi: 0.346
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.42
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TCTCCTTTTGTGTTAAGCTTCT	TTGATTCTTCGTCGCCGTAG	36.4	50.0	55.6	58.1	2173 bp	0.043478	5.6
rps19-rpl2_p2	TCTCCTTTTGTGTTAAGCTTCT	TTGGGGTTATCCTGCACTTG	36.4	50.0	55.6	57.8	2231 bp	0.043478	4.7
rps19-rpl2_p3	TCTCCTTTTGTGTTAAGCTTCT	ATTTGATTCTTCGTCGCCGT	36.4	45.0	55.6	58.3	2175 bp	0.043478	4.2
rps19-rpl2_p4	TCTCCTTTTGTGTTAAGCTTCT	TTTGATTCTTCGTCGCCGTA	36.4	45.0	55.6	57.6	2174 bp	0.043478	3.6
rps19-rpl2_p5	TCTCCTTTTGTGTTAAGCTTCTT	TTGATTCTTCGTCGCCGTAG	34.8	50.0	56.3	58.1	2173 bp	0.043478	3.5