Hotspot

trnQ-UUG-accD

Genome · 57308-57919 · 612 bp

Back to Caragana

Pi 0.0391

MarkerSeek score 76.40

Primer available yes

Label trnQ-UUG-accD

Feature Metadata

label_name: trnQ-UUG-accD
feature_type: igs
region: Genome
length_bp: 612
variable_sites: 103
pi: 0.0391
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-accD_p1	GAGGTTCGAATCCTTCCGTC	TCCTGAAATAATCAACATTGCTGT	55.0	33.3	58.1	57.2	654-713 bp	0.956522	50.9
trnQ-UUG-accD_p2	GAGGTTCGAATCCTTCCGTC	CCTGAAATAATCAACATTGCTGT	55.0	34.8	58.1	55.8	653-712 bp	0.956522	50.3
trnQ-UUG-accD_p3	GAGGTTCGAATCCTTCCGTC	TGTTCCTGAAATAATCAACATTGCT	55.0	32.0	58.1	57.8	657-716 bp	0.956522	50.3
trnQ-UUG-accD_p4	GAGGTTCGAATCCTTCCGTC	TTCCTGAAATAATCAACATTGCTGT	55.0	32.0	58.1	57.8	655-714 bp	0.956522	50.3
trnQ-UUG-accD_p5	GAGGTTCGAATCCTTCCGTC	CCGTATGTTCCTGAAATAATCAACA	55.0	36.0	58.1	57.7	662-721 bp	0.956522	50.3