Hotspot

trnG-UCC-trnS-GCU

Genome · 55046-55546 · 501 bp

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Pi 0.0164
MarkerSeek score 75.80
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
Genome
length_bp
501
variable_sites
52
pi
0.0164
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 GGGGTCTATAGAACGAATCGC AATCCGACGCTTTAGTCCAC 52.4 50.0 58.1 58.0 484-618 bp 1.0 82.8
trnG-UCC-trnS-GCU_p2 ACGAATCGCACTTTTACCACT AATCCGACGCTTTAGTCCAC 42.9 50.0 58.2 58.0 472-606 bp 1.0 81.3
trnG-UCC-trnS-GCU_p3 GGGGTCTATAGAACGAATCGC ACGGAAAGAGAGGGATTCGA 52.4 50.0 58.1 58.2 538-672 bp 1.0 81.0
trnG-UCC-trnS-GCU_p4 GGGGTCTATAGAACGAATCGC CAATCCGACGCTTTAGTCCA 52.4 50.0 58.1 58.0 485-619 bp 0.956522 79.5
trnG-UCC-trnS-GCU_p5 ACGAATCGCACTTTTACCACT CAATCCGACGCTTTAGTCCA 42.9 50.0 58.2 58.0 473-607 bp 0.956522 78.2