Pi
0.0287
MarkerSeek score
72.50
Primer available
yes
Label
trnL-CAA-trnT-UGU
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnL-CAA-trnT-UGU_p1 | ACCAATTTCGCCATATCCCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.0 | 57.9 | 1315-1771 bp | 1.0 | 90.9 |
| trnL-CAA-trnT-UGU_p2 | TAGCGTCTACCAATTTCGCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.1 | 57.9 | 1323-1779 bp | 1.0 | 90.4 |
| trnL-CAA-trnT-UGU_p3 | ACCAATTTCGCCATATCCCC | GGAATCGAACCGATGACCAT | 50.0 | 50.0 | 58.0 | 57.8 | 1347-1803 bp | 1.0 | 90.0 |
| trnL-CAA-trnT-UGU_p4 | TAGCGTCTACCAATTTCGCC | GGAATCGAACCGATGACCAT | 50.0 | 50.0 | 58.1 | 57.8 | 1355-1811 bp | 1.0 | 89.5 |
| trnL-CAA-trnT-UGU_p5 | CCCATTGTGAATTCCAGGGT | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 57.8 | 57.9 | 1408-1864 bp | 1.0 | 89.0 |