Pi
0.00475
MarkerSeek score
41.70
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | ACAGATGTCGTTTCTAGTCT | GCGGACGCATCTCTTCTTAT | 40.0 | 50.0 | 53.5 | 57.9 | 1253-1393 bp | 1.0 | 47.2 |
| ycf1_p2 | AACAGATGTCGTTTCTAGTCT | GCGGACGCATCTCTTCTTAT | 38.1 | 50.0 | 54.4 | 57.9 | 1254-1394 bp | 1.0 | 46.3 |
| ycf1_p3 | AGAAATAAGGGAGGTTTGTG | GCGGACGCATCTCTTCTTAT | 40.0 | 50.0 | 52.7 | 57.9 | 1158-1297 bp | 1.0 | 44.6 |
| ycf1_p4 | ACAGATGTCGTTTCTAGTCTATCTC | GCGGACGCATCTCTTCTTAT | 40.0 | 50.0 | 57.7 | 57.9 | 1253-1393 bp | 1.0 | 44.6 |
| ycf1_p5 | ACAGATGTCGTTTCTAGTCTATCT | GCGGACGCATCTCTTCTTAT | 37.5 | 50.0 | 56.7 | 57.9 | 1253-1393 bp | 1.0 | 44.6 |
| ycf1_p1 | GAAATGGTCGATTCCACCGA | TCACCTTACAGAGAGAGGCT | 50.0 | 50.0 | 58.0 | 57.1 | 215-233 bp | 1.0 | 78.7 |
| ycf1_p2 | GAAATGGTCGATTCCACCGA | CTCACCTTACAGAGAGAGGCT | 50.0 | 52.4 | 58.0 | 58.3 | 216-234 bp | 1.0 | 75.9 |
| ycf1_p3 | ACGGCTTGGTCAAATCGATT | TCACCTTACAGAGAGAGGCT | 45.0 | 50.0 | 58.2 | 57.1 | 249-267 bp | 0.933333 | 72.4 |
| ycf1_p4 | GAAATGGTCGATTCCACCGA | CTCACCTTACAGAGAGAGGC | 50.0 | 55.0 | 58.0 | 56.8 | 216-234 bp | 0.933333 | 71.5 |
| ycf1_p5 | GAAATGGTCGATTCCACCGA | TCTCACCTTACAGAGAGAGGC | 50.0 | 52.4 | 58.0 | 58.3 | 217-235 bp | 0.933333 | 71.3 |