Hotspot

rps19-rpl2

IRb · 87433-87508 · 76 bp

Back to Capsicum

Pi 0.0103

MarkerSeek score 55.70

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 76
variable_sites: 4
pi: 0.0103
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCCCGGGACCAAGTTACTAT	TTGATTCTTCGTCGCCGTAG	55.0	50.0	59.2	58.1	199-213 bp	1.0	75.4
rps19-rpl2_p2	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	55.0	55.0	59.2	58.2	266-280 bp	1.0	74.6
rps19-rpl2_p3	GCCCGGGACCAAGTTACTAT	TTGGGGTTATCCTGCACTTG	55.0	50.0	59.2	57.8	257-271 bp	1.0	74.2
rps19-rpl2_p4	GCCCGGGACCAAGTTACTAT	ATTTGATTCTTCGTCGCCGT	55.0	45.0	59.2	58.3	201-215 bp	1.0	74.0
rps19-rpl2_p5	CCCGGGACCAAGTTACTATT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	56.6	58.1	198-212 bp	1.0	73.7