Hotspot

psaJ-rpl33

LSC · 70336-70771 · 436 bp

Back to Capsicum

Pi 0.00546

MarkerSeek score 49.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 436
variable_sites: 7
pi: 0.00546
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	CTCGGACATCTTTCCCCTTG	45.0	55.0	58.1	58.0	481-493 bp	1.0	83.2
psaJ-rpl33_p2	GGCACCAGTACTAAGTACGC	CTCGGACATCTTTCCCCTTG	55.0	55.0	58.1	58.0	555-567 bp	1.0	82.6
psaJ-rpl33_p3	TGCGTTGACATTCCCCTTTT	CTCGGACATCTTTCCCCTTG	45.0	55.0	58.3	58.0	480-492 bp	1.0	81.4
psaJ-rpl33_p4	ATCTCTCTGTGGCACCAGTA	CTCGGACATCTTTCCCCTTG	50.0	55.0	57.8	58.0	565-577 bp	1.0	81.3
psaJ-rpl33_p5	TATCTCTCTGTGGCACCAGT	CTCGGACATCTTTCCCCTTG	50.0	55.0	57.8	58.0	566-578 bp	1.0	81.3