Hotspot

ndhC-trnV

LSC · 52679-53814 · 1136 bp

Back to Capsicum

Pi 0.00379

MarkerSeek score 52.60

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 1136
variable_sites: 23
pi: 0.00379
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	TTGCTAATCGGGGCTAACAC	CGGTTCAAGTCCGTATAGCC	50.0	55.0	58.0	58.2	1251-1273 bp	1.0	82.8
ndhC-trnV_p2	TTGCTAATCGGGGCTAACAC	GGTTCAAGTCCGTATAGCCC	50.0	55.0	58.0	57.8	1250-1272 bp	1.0	82.1
ndhC-trnV_p3	GCTAATCGGGGCTAACACTC	CGGTTCAAGTCCGTATAGCC	55.0	55.0	58.1	58.2	1249-1271 bp	1.0	82.0
ndhC-trnV_p4	GCTAATCGGGGCTAACACTC	GGTTCAAGTCCGTATAGCCC	55.0	55.0	58.1	57.8	1248-1270 bp	1.0	81.3
ndhC-trnV_p5	CCTTTGCTAATCGGGGCTAA	CGGTTCAAGTCCGTATAGCC	50.0	55.0	57.7	58.2	1254-1276 bp	1.0	80.3