Hotspot

trnF-ndhJ

LSC · 50329-50999 · 671 bp

Back to Capsicum

Pi 0.0059

MarkerSeek score 50.70

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 13
pi: 0.0059
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	AATAGCCGGGATAGCTCAGT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	816-820 bp	1.0	81.4
trnF-ndhJ_p2	ATAGCCGGGATAGCTCAGTT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	815-819 bp	1.0	81.4
trnF-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	773-777 bp	1.0	81.3
trnF-ndhJ_p4	GGTTACCAGTCCCAACCTTG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.1	58.1	855-859 bp	1.0	80.7
trnF-ndhJ_p5	ATAGCCGGGATAGCTCAGTT	ATCATCCACGCTTGAAACGT	50.0	45.0	58.0	58.2	850-854 bp	1.0	80.5