Hotspot

rps4-trnT

LSC · 47998-48373 · 376 bp

Back to Capsicum

Pi 0.0137

MarkerSeek score 56.20

Primer available yes

Label rps4-trnT

Feature Metadata

label_name: rps4-trnT
feature_type: igs
region: LSC
length_bp: 376
variable_sites: 24
pi: 0.0137
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT_p1	GATCATTTCCGGTCCTAGGC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.8	57.9	533-538 bp	1.0	83.1
rps4-trnT_p2	ATCATTTCCGGTCCTAGGCT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.2	57.9	532-537 bp	1.0	83.0
rps4-trnT_p3	GATCATTTCCGGTCCTAGGC	AGATTTCTGTGATGTGGGCC	55.0	50.0	57.8	57.9	560-565 bp	1.0	82.8
rps4-trnT_p4	ATCATTTCCGGTCCTAGGCT	AGATTTCTGTGATGTGGGCC	50.0	50.0	58.2	57.9	559-564 bp	1.0	82.7
rps4-trnT_p5	GATCATTTCCGGTCCTAGGC	ATGGTCATCGGTTCGATTCC	55.0	50.0	57.8	57.8	501-506 bp	1.0	82.6